Synthesis and Analysis of Peptide Ligand for Biosensor Application Using Combinatorial Chemistry: Screening and Characterization of 2,3,7-Trichlorodibenzo-p-dioxin-Binding Peptide

摘要

Dioxins and endocrine disrupting chemicals are toxic and bad for health, and they become a one of social problem. The combinatorial synthesis of chemical libraries by the split-and-mix strategy makes it easy to synthesize a large number of peptide libraries and possible to select an optimal peptide that binds with chemicals. In this study, the screening and the characterization of peptide ligands that bind with high affinity to 2,3,7-trichlorodibenzo-p-dioxin (2,3,7-TCDD) were carried out. Combinatorial peptide libraries were synthesized by solid phase synthesis and they consisted of hepta-peptide sequences which bind to resin with the C-terminal of the peptide. Five peptides which bound with 2,3,7-TCDD were screened from peptide library, and amino acid sequences of these peptides were investigated. One of the peptides, designed as peptide A, as the concentration of 2,3,7-TCDD increased, the fluorescence decreased almost proportionally in the competitive binding assay. This indicated that the analysis system could be used for detection of 2,3,7-TCDD.