Prediction of Differentiation-ralated Pathways Induced by Rho-GDIγ

摘要

Rho-GDIγ, one of GDP-dissociation inhibitors (GDIs) in the GTPases Rho-subfamily, plays a key role in modulating the activation of GTPases, and contributes to reorganization of actin cytoskeleton, cell aggregation, cell-to-cell adhesion, membrane ruffling, cell motility, and so on. In this work, a functional linkage network that consists of genes differentially expressed between undifferentiated and differentiated neural stem cells (NSCs) is first constructed. Differentiation-ralated pathways induced by Rho-GDIγ are subsequently predicted from the functional linkage network. The results of the biological experiments indicated that overexpression of Rho-GDIγ in neural stem cell line C17.2 upregulates VCP (valosin-containing protein) gene. The results demonstrate that differentiation-related pathways predicted from functional linkage network are indeed reliable.