Electron and scanning probe microscopy study of S-layers of plague microbes

摘要

Abstract: Microscopy investigations of S-layers of plague microbes have been carried out. A protein forming S-layer was isolated, purified and its biochemical properties were studied. Images of plague cells with and without S-layer and images of isolated S-layer protein arrays on solid supports were obtained by transmission electron microscopy. An isolated protein forming the layer is self-assembled into crystalline structure with hexagonal pores are of regular size about 4 - 8 nm, which was observed by transmission electron micrographs. The STM images of plague microbes with S-layer and without one and the STM and AFM images of isolated S-layer protein arrays on mica surface were obtained. !11