New light induced molecular switch allows sterically oriented micrometer sized immobilization of biomolecules

摘要

Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized spots. The methodology is considered to be a strong alternative to the conventional procedures which often include the use of harsh conditions such as strong chemicals and elevated temperatures. The technology behind this immobilization technique -here termed "light assisted immobilization" - is based on the fact that disulphide bridges that are naturally present within the protein structure can be broken as a result of UV-illumination. The free thiol groups (-SH) created upon disruption of a disulphide bridge are very reactive and can be used as linkers for covalent attachment to a surface. The surface can for example be gold or thiol-derivatized silicon, making this technology extremely useful for a large range of application areas, including biosensors.