Prediction of Bovine Cartilage Proteoglycan Content Using Energy Dispersive X-ray Analysis or Optical Absorbance and a Multivariate Techniques-Fourier Transform Infrared Microspectroscopy Model

摘要

Two analytical techniques: optical absorbance of Safranin-O stained cartilage sections and energy dispersive X-ray analysis have been used to construct partial least squares models from Fourier transform infra red spectral data. These models can be used to semi-quantitatively predict proteoglycan content in cartilage sections. Valuable information about the spatial distribution of the constituents in native, degraded or even engineered cartilage, can be obtained when these are used in conjunction with infra red imaging techniques.