Determining Elasticities in situ

摘要

Knowledge of in situ enzyme kinetics is an important aspect of metabolic regulation. Reaction kinetics is usually studied in vitro using enzymes that have been isolated from the organism by biochemical methods, even theough it is known that such in vitro data may have little relevance in situ. Recently, this old and famous dilemma of enzyme kinetics has won new relevance by the needs of metabolic engineers for advice as to how the kinetics should bemodified in order to reach a specific goal, such as overproduction of a componud. However, it is not only for the applied aspects that knowledge of the insitu dinetics of metabolic systems is essential. In situ kinetics is also an important and interesting topic in its own right. Among others, it helps us to find out whether our present understanding of enzyme kinetics is adequate to describe the in vivo situation.