Combined NMR Experimental and Computer-Simulation Study of 2,3-Bisphosphoglycerate Metabolism in Human Erythrocytes

摘要

We have developed a new model of the metabolism of the human erythrocyte that is sufficiently comprehensive to explain the responses under many reproducible experimental conditions. The task has been addressed extensively in previous publications (Rapoport et al., 1976; Heinrich et al., 1977; Schuster et al., 1988; Johi & Palsson, 1989; Schuster & Holzhutter, 1995) but all earlier models fall short of providing a satisfactory comparison between their predictions and experimental data on carbon and phosphate flux into and out of the Rapoport-Luebering shunt, glycolysis and the phosphate flux into and out of the Rapoport-Luebering shunt, glycolysis and the pentose phosphate pathway, theat can be obtained by using ~1H, ~(13)C and ~(31)P NMR spectroscopy (Oxley et al., 1984; Thorburn & Kuchel, 1985; McIntyre et al., 1989; berthon et al., 1993). On the other hand, the previous models did serve to identify the many features of the regulation and control of glycolysis and were instrumental in the development of metabolic control theory (Heinrich et al., 1977).