A Multiple Drugs of Abuse Test for Saliva Specimen on a Single Lab-on-a-Film-Chip Using Electrochemical Immunoassay

摘要

A lab-on-a- film-chip (LOFC) developed by Nano-Ditech Corp is a disposable microfluidic immunoassay device. Multiple drugs of abuse test for saliva specimen has been developed on a single lab-on-a-film-chip as an alternative to a nitrocellulose membrane based immunochromatography assay. The assay is a competitive test format since the target analytes have small molecular weights. The drug-conjugates immobilized on a carbon electrode compete with free drugs in saliva specimens for a binding site of specific drug antibody conjugated with alkaline phospatase. The binding signal for each analyte is generated by captured antibody conjugated with APase on a separate carbon electrode and is analyzed by electrochemical assay. Three analyte, amphetamine, cocaine and opiate, are simultaneously detected from drug spiked saliva samples with the cutoffs of 20 ng/ml, 40 ng/ml and 50 ng/ml, respectively.