Modified Nanoparticle Films for Transmission Plasmon Biosensing

摘要

The principle of the Transmission Plasmon Biosensor (TPB) is based on the Localized Surface Plasmon Resonance (LSPR) properties of noble metal nanoparticles attached to a transparent substrate. When the analyte of interest binds to the biological receptor molecules immobilized onto these nanoparticles, the absorption of the nanoparticles will change due to local changes in the refractive index (RI). In this study, we investigated various parameters in order to increase the final sensitivity of the TPB such as the deposition method, the size, the material and the (bio)functionality of the nanoparticles. For biosensing applications, the nanoparticles were further functionalized with the desired biological receptor molecules (e.g. antibodies or ampicillin) using mixed Self-Assembled Monolayers (SAMs) [1]. In this paper, the experimental results on the modification and the characterization of the nanoparticle films will be presented together with the preliminary results on biological sensing.