Development of Micro-Lateral Flow Strips Compatible with Micro-Volume Samples for Immunochemical Assays

摘要

MicroPlumbers Microsciences LLC has developed elements of a miniaturized lateral flow strip (LFS) based device that is compatible with low-pain microvolume blood extraction methods having sample volumes as small as 1 microliter. A related device design with parallel configuration of miniaturized LFS that performs 10 independent assays from a single drop of sample (approx. 25 microliters) has also been demonstrated. The elements of a miniaturized LFS including application, capture, control and absorber pads were fabricated from backed sheets of nitrocellulose by milling on a computer numeric control table top milling machine. The functionality of these miniaturized LFSs has been demonstrated using avidin and troponin I antibody modified microspheres and the appropriate ligands immobilized at capture pads of the miniaturized LFS.