Fabrication of molecularly imprinted uric acid biosensors based on a novel amine-imide type conducting polymer

摘要

In this study, a molecularly imprinted polymer (MIP) based uric acid sensor was fabricated using a novel amine-imide type conducting polymer. Calibration curves of the sensor were depicted based on the amperometric steady-state current in different concentrations of UA ranging from 0 to 1.125 Mm. The sensitivities of MIP and non-MIP electrodes were calculated to 24.72 and 6.63 Μa Mm~(-1)cm~(-2), respectively. The imprinting efficiency, defined as the ratio of the sensitivity of MIP to that of NMIP electrode, was 3.7 in this work. The limit of detection (LOD) for the MIP-ITO/Poly(PD-BCD)_(UA) electrode was calculated to be 2.4 × 10~(-3)Mm on the basis of signal to noise ratio (S/N) equaling to 3. It was found that the MIP sensor showed relatively good sensitivity, large linear range, and high imprinting efficiency with a low LOD for sensing UA. The MIP-ITO/Poly(PD-BCD)_(UA) electrode was also tested for ascorbic acid (AA), and the current response of 0.04 Mm AA is less than 7 % of the current response of 0.4 Mm UA.