Assembly of DNA Rotaxanes for AFM Based Sequencing

摘要

Development of DNA sequencing technologies has been fueled by the desire to unlock the genetic information stored inside of genomes. This information can help fields as varied as molecular medicine, bioarcheology, forensics, and environmental science. The challenge is to establish a method that is more cost effective and faster than the ones currently available. Our technique takes advantage of the atomic force microscope's (AFM) ability to detect piconewton scale forces in order to read off sequencing information based on the interaction of nucleotides with a cyclodextrin.