In this talk, I will discuss quantitative biology and medicine by nanobiophotonics and BioASICs. Using new paradigms of biological inspiration and understanding of electron transfer mechanism in biological systems, we have created quantized Plasmon Resonance Energy Transfer (PRET) nanospectroscopy for molecular imaging of living cells (Fig. 1). For the remote optical control of gene regulation and protein expression, we have developed Oligonucleotides on a Nanoplasmonic Carrier Optical Switch (ONCOS). ONCOS allows on-demand gene silencing with nanometer-scale spatial resolution and localized temperature disturbance in living cells. The ONCOS and PRET will be used for experimental system biology, molecular/cellular diagnostics, and therapeutic applications since it will provide us precise spatial and temporal information of living cellular mechanism. Bionanophotonic molecular ruler is accomplished to measure the dynamics of DNA and protein interactions. In-vivo Surface Enhanced Raman Spectroscopy (SERS) probes, in-vitro integrated nanofluidic SERS, and optofluidic microprocessors are developed for label-free molecular diagnostics and drug discovery. In order to accomplish physiologically relevant cell culture platforms, we have developed Biological Application Specific Integrated Circuits (BioASICs) for patch clamp array (Fig. 2) single cell biophysics, quantitative cell biology, and cell-based diagnostics by connecting novel microfluidics and nanofluidic circuits, which can impact on high-speed and high-content repeatable biology, quantitative medicine, and biophysics in new ways. We are creating a library of these "building blocks" to develop innovative single cell array, physiologically relevant dynamic cell culture array (Fig. 3), and biological microprocessors with integrated optical controls and detections capability.
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