Metabolic Engineering of Bacteria for the Production of alpha-olefins

摘要

1. Cyanobacterial olefins can be synthesized 1.1 By polyketide-like, olefin synthases 1.2By P450 hydroxylase/decarboxylase 1.3 Protein engineering will be used to alter the substrate specificity and make olefins 2. Translational coupling as a Met Eng tool 2.1 Useful for detecting full translation 2.2 Useful for optimizing expression conditions 2.3 Qualitatively proportional over wide range 2.4 Useful for keeping stoichiometric ratios of proteins?