Liquid chromatographic method for determination of four active saponins from Panax notoginseng in rat urine using solid-phase extraction

摘要

Four major active saponins (ginsenosides Rg_1, Rb_1 Rd and notoginscnoside R_1) in Panax notoginseng were determined in rat urine after oral and intravenous administration of total saponins of P. notoginseng (PNS), and the urine samples were treated with solid-phase extraction (SPE) prior to liquid chromatography. A reversed-phase liquid chromatography system with ultraviolet detection and a Zorbax SB-C_(18) column was used. The within-day and between-day assay coefficients of variation for the four saponins in urine were less than 7% and the recovery of this method was higher than 85%. Using this method, the excretion profile of the drug in rat urine after administration of PNS was revealed for the first time.