Deposition and Persistence of Polyanhydride Nanoparticle Vaccines upon Intranasal Administration

摘要

Particle size was shown to significantly impact deposition within the lungs upon intranasal administration, with the monodisperse 470 nm nanoparticles having the greatest deposition. The persistence of the nanoparticles in the lung is dependent upon polymer chemistry, with the more readily erodible poly(SA) particles being cleared within 48 hours of administration. In contrast, the slower degrading 50:50 CPTEG:CPH nanoparticles persisted longer and greater biodistribution was observed. The delivery of the antigen in conjunction with traditional adjuvants like MPLA was quickly cleared from the lungs. The persistence of polyanhydride nanoparticles sustains antigen release, resulting in continuous recruitment of APCs. The greater persistence of the 50:50 CPTEG:CPH nanoparticles in the lungs may explain the previously observed long-lived (23 weeks) anti-F1-V antibody titer and protective immunity against pneumonic plague in mice immunized with F1-V encapsulated into polyanhydride nanoparticles [3].