Bioactivity of a multivalent cell membrane binder in 3D spheroid culture: effects of RGD-dendrimer conjugate on cell proliferation, expression and aggregation

摘要

Multivalent adhesive conjugates were incorporated into the multicellular aggregates in a dosage controllable manner. Under 3D culture conditions, the RGD-PAMAM conjugates were capable of promoting multicellular aggregation and enhancing the cellular activity and function in vitro. Ligand modified dendrimers therefore may have potential for use as soluble functional nanomaterials to provide new methodologies for 3D cell culture and tissue engineering.