A Redox-Enzyme-Based Electrochemical Biosensor with a CMOS Integrated Bipotentiostat

摘要

This paper presents an electrochemical biosensor featuring redox enzymes and a supporting CMOS bipotentiostat. The bipotentiostat architecture supports redox recycling through a common potential control unit and two readout channels where excitation signals can be inserted. The readout channel was characterized to have a dynamic range from 1 nA to 10 μA. A fructose sensor was created on a microfabricated interdigitated electrode array as an example redox-enzyme-based biosensor. Cyclic voltammogram measurements show a 27% increase in electrochemically magnified current due to redox recycling while measuring 200 mM fructose. Chronoamperometry experiments using the CMOS bipotentiostat agree well with a commercial bipotentiostat for fructose concentration from 50 mM to 400 mM.