Enhanced Gene Delivery Using Listeriolysin O-Protamine Fusion Protein

摘要

To improve the gene transfection efficiency of nonviral vectors by overcoming important barriers, a bifunctional fusion protein composed of the endosomolytic protein listeriolysin O (LLO) at the Nterminus and a DNA-binding peptide fragment of protamine (PN) at the C-terminus was recombinantly expressed. Through the use of LLO-PN fusion protein, transfection efficiency was greatly enhanced, suggesting the improved gene delivery ability of this non-viral vector.