Development of EST-SSR Markers in Pleurotus ostreatus and Construction of Primary Core Collection

摘要

Based on the EST sequences published on NCBI, 9 Pleurotus ostreatus EST-SSR primer pairs, 58 Ganoderma lucidum EST-SSR primer pairs and 35 Lentinula edodes EST-SSR primer pairs were designed. Among these, 4 with good polymorphisms and clear distinctions were selected to analyze 185 strains of Pleurotus ostreatus. After amplification, 59 alleles were generated, 12-19 alleles for each primer, with the fragment sizes between 100 bp and 1 100 bp. A primary core collection, P-core 47, composed of 47 accessions was constructed based on the amplification results. Analysis identified that the P-core 47 accounted for 25 % in the original sample pool and 13 % in the original resource pool. As for the number of effective alleles and the ratio of polymorphic sites, consistency was preserved between P-core 47 and the original pool. In addition, compared to the original pool, diversity index of Pcore outperformed by 0. 12747, and alleles used to be of low frequency in the original pool were strengthened as well. The strains in P-core 47 are broadly distributed, available in most cultivation regions. Indeed you can find representative alleles associated with both qualitative and quantitative traits in this collection. This study showed the feasibility of adopting EST-SSR markers to construct a primary core strain collection of the Pleurotus ostreatus.