DNA2.0 has discovered, characterized and engineered new transposases that work comparable to, or better, than previously commercialized transposases. The technology is highly valuable for protein expression and genome engineering applications. The Leap-In Transposase? is an engineered enzyme that catalyzes the integration of a transposon and your gene of interest into TTAT sites in the target genome. The technology enables a specified sequence of interest to behave as a transposon, a mobile genetic element, which can efficiently transpose between vectors and chromosomes via a “cut & paste” mechanism. During transposition, the Leap-In Transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and moves the contents from the original sites and efficiently integrates them into TTAT chromosomal sites. Similar technologies report integration of up to 20 copies of the gene of interest into unique locations in the genome 72 hours post transfection. Furthermore, transposase technologies are highly valuable because of their ability to integrate large payloads. This technology significantly accelerates stable pool and cell-line generation.
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