A Bispecific Antibody Generated with Sortase and Click-Chemistry Has Broad Anti-Influenza Virus Activity

摘要

Bispecific antibodies have therapeutic potential by expanding the functions of conventional antibodies. Many different formats of bispecific antibodies have meanwhile been developed. Here, we present a new bispecific format, AIMLink, where we have fused two full-sized IgG antibodies via their C-termini utilizing sortase-mediated transpeptidation and click-chemistry to create a covalently linked IgG antibody heterodimer. By linking two potent anti-influenza A antibodies together we have generated a full antibody dimer with bispecific activity that retains the full activity and stability of the two fusion partners.