Nanoparticle Peptide Synthesis NPPS

摘要

Nanoparticles (NP) modification techniques are mostly based on the conjugation of a single molecule (protein etc.) by covalent bonding of complementary functional groups found on both components. Because of the difficulty in isolating NPs by filtration/precipitation or dialysis, conjugation is mostly restricted to a single or few coupling reactions due to the difficult removal of excess reagents after every reaction. Our NPs possess both bio and chemical compatibility; these qualities rend their modification a difficult task due to non-aggregative behavior that prevents their precipitation. This physical difficulty was overcome by a new approach that exploits magnetic susceptibility of materials for their easy and fast separation. The presented method might be applied to other NPs, thus extending the scope of Merrifield synthesis to the nanometric range.