Isolation of Endophytic Fungi from Stem of Andaleh (Morus macroura Miq.) That Produce Antimicrobial Compound

摘要

Antibiotic resistance is a serious problem that needs to be dealt with immediately. One of solution can be issued is find and develop new potential antimicrobial derived from natural resources. Stem of Andaleh plant (Morus macroura Miq.) is potential candidate of natural resources which has antimicrobial activity, but it was an endangered species, so using this plant as antibiotic source is not wise. We can use endophytic fungi from Andaleh's plant since they are known have the ability to produce similar antibiotic compound. So, the research aiming is to find potential candidate of endophytic fungi that have ability to produce antimicrobial compound. Stem sample of Andaleh are from Batusangkar. Stem surface sterilization was using NaOCl 0.5%. The isolation of endophytic fungi from Andaleh stem was using PDA containing Chloramphenichol 500mg/l. Antimicrobial activity assay was conducted by agar dilution method using 3 microbial agent i.e. Escherichia coli, Staphylococcus aureus and Candida albicans. There are 7 pure isolate of endophytic fungi from Andaleh stem. All of isolate, have antimicrobial activity against E. coli and S. aureus, but none of them have inhibitory effect to C. albicans. Antimicrobial activity index against E. coli ranging from 2.51 to 5.71 which the highest index was from isolate 1. Meanwhile antimicrobial activity index against S. aureus ranging from 1.35 to 4.07 which the higest index was from isolate 2. So, it can be concluded that endophytic fungi from stem of Andaleh are potential to produce antimicrobial compound and can be developed to a new antibiotic source.