Development of electrochemical biosensor for breast cancer detection using gold nanoparticle doped CA 15-3 antibody and antigen interaction

摘要

The diagnosis of breast cancer in the current scenario is by Mammogram, MRI and its spectroscopy and breast biopsy test. All these detection techniques are very costly, involve surgical techniques, time-consuming and results are sometimes negative. Hence, there is a need to develop a cost effective technique that can qualitatively and/or quantitatively detect the breast cancer at an early stage. Hence the development of a highly sensitive sensor system for monitoring the breast cancer is required so that the patient can remain alert for the risk of getting breast cancer and the level of re-occurrence. This project aims to develop a label-free electrochemical immunosensor based on the CA 15-3 level in serum and the cancer marker interaction with specific antibody coated with gold nanoparticle. These nanoparticles exerts higher surface area for enhanced reaction site and higher electrical conductivity that can provide detectable signals for the sensor which can be read using a electrochemical impedance spectroscopy (EIS) and potentiostat.