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Study of 4,4- Dithiodibutyric Acid as a Monolayer for Protein Chip

机译:4,4-二碘丁酸作为蛋白质芯片单层的研究

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Despite considerable efforts, the fabrication of protein chips using a self-assembled monolayer (SAM) with a long chain length remains a challenge, due to its steric hindrance and disulfide deposited, which can generate multilayers and block the functional groups of the SAMs, and thus, significantly reduce the sensitivity of the chips. To improve their sensitivity, the feasibility of using a short-chain SAM, 4,4-dithiodibutyric acid (4,4-DTBA), as a monolayer for the protein chips based on a gold surface is studied. Experiments for characterizing 4,4-DTBA are performed by atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and ellipsometry. Additionally, the fluorescent assay of 4,4-DTBA is characterized using protein A-FITC (fluorescein isothio cyanate) to investigate its binding capacity. The results of 4,4-DTBA are compared with those of 11-MUA (11-mercapto-undecanoic acid). The comparison results indicate that 4,4-DTBA can be adopted as a monolayer for the protein chips.
机译:尽管努力具有相当大的努力,由于其空间障碍和二硫化物,但是使用具有长链长度的自组装单层(SAM)的蛋白质芯片的制造仍然是挑战,这可能产生多层并阻止SAM的官能团和块因此,显着降低了芯片的敏感性。为了提高它们的敏感性,使用短链SAM,4,4-二碘丁酸(4,4-DTBA)的可行性,作为基于金表面的蛋白质芯片的单层。用原子力显微镜(AFM),傅里叶变换红外光谱(FTIR)和椭圆形来进行表征4,4-DTBA的实验。另外,4,4-DTBA的荧光测定用蛋白A-FITC(荧光素Isothio Cyanate)的特征表征,以研究其结合能力。将4,4-DTBA的结果与11-Mua(11-巯基 - 过癸酸)进行比较。比较结果表明,4,4-DTBA可以用作蛋白质芯片的单层。

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