Study of Artemisia annua Proteome for Presence of Metal-Dependent Proteins in Intrusion Zone to Agrobiocenosis

摘要

The article presents the results of study metal-dependent proteins comprised in Artemisia annua proteome in the intrusion territories adjacent to the artificial agrobiocenosis in Volgograd region. Using mass spectrometry we detected more than 2200 proteins, including 134 metal-dependent ones. One hundred twenty one of them had a dependence on zinc, 9 proteins depended on copper, and 4 proteins were manganese-dependent. We found no any nickel-or cobalt-dependent proteins. Zinc-dependent proteins mainly worked in gene expression and energy metabolism regulation. Copper-dependent proteins involved in enzymatic reactions. Manganese-dependent proteins perform functions of DNA repair and enzymatic hydrolysis. According to these results, zinc-dependent proteins are the most prevalent from metal-dependent proteins of Artemisia annua in the intrusion territories adjacent to the artificial agrobiocenosis of Volgograd region. Further study can stimulate the development of point control methods to reduce Artemisia population and its effect from the technogenic intrusion zone to adjacent agrobiocenosis.