Molecular Damage Evaluation and Chromosome Aberration in Blood Lymphocyte of Medical Radiation Workers

摘要

A preliminary evaluation on the cytogenetic effects and DNA damages was done in blood of twenty two person at the age between 30-56 years old from medical workers as expose group and their matched twenty from administration workers (control). Their bloods were collected with their full informed consent and culture set up for cytogenetic evaluation as well as nucleic acid damage observation with comet assay that were done according to the standard procedures. Lymphocytes were scored, either by using analysis of manual observation and automatically with metaphase finder, for the presence of chromosomal aberration and Mitotik Index. The result show that the Mitotik Index on expose group were no significant differences between exposure group and control. The frequencies of disentric chromosomes both in expose group and control were not found. Evaluation of DNA damage observation using Comet assay showed no any effects observed in lymphocytes for both study and control groups. Our data did not reveal any significant difference in expose group compared to control level in parameters by mean cytogenetics biomarkers studied which is supported by the Comet evaluation.