Enhancement of Monoclonal Antibody Production under High Osmotic Pressure

摘要

Based on the study respect to energy metabolism under high osmotic pressure and cell adaptation to the increase in osmotic pressure, a strategy of gradual increase in osmotic pressure was applied in monoclonal antibody(MAb) production. The specific production rate of MAb(q_MAb) and MAb productivity at 388 mOsmol/kg were about 2 and 1.5 times higher, respectively, than those at the physiological osmotic pressure. The average specific rate of glucose consumption(V_G) increased with osmotic pressure until 410 mOsmol/kg and then decreased. The specific consumption rate of glutamine(V_GLN) increased slowly below 410 mOsmol/kg but rapidly above it.