Rapid and sensitive strategy for Human Papillomavirus (HPV) detection using a gene-based DNA nanobiosensor

摘要

Human Papillomavirus (HPV) is the most common sexually transmitted virus that infected to human. The most common high-risk HPV are 16 and 18. Most 70% of cervical cancers (CC) are due to infection by these genotypes. A rapid and sensitive strategy for the detection of Human Papillomavirus (HPV) was proposed by integrating simple DNA extraction with gene-based DNA nanobiosensor. DNA nanochip is a powerful tool allowing detection HPV target molecules present in a sample. The efficiency of DNA nanochip depends mainly on the sequence of the capture probes and the way they are attached to the support. The coupling procedure must be quick, covalent, and reproducible. The fabrication, immobilization and hybridization processes were characterized with current voltage (I-V) characterization (KEITHLEY, 6487). This strategy presented a simple, rapid and sensitive platform for HPV detection and would become a powerful tool for pathogenic microorganisms screening in clinical diagnosis.