5-ALA induces the distribution of endogenous PpIX fluorescence in cervical lesions

摘要

Objective: To investigate the distribution of ALA in Cervical lesion tissue by tissue frozen section and fluorescence microscopy. Methods: 20% of 5-ALA were topically applied to the cervix.Biopsies were taken from the lesions for histology examination,Fluorescence microscopy was performed methods in these frozen section of cervical tissue to observe the distribution of ALA and the result was analyzed semi-quantitatively. Results : The maximal Protoporphyrin IX (PpIX ) fluorescence was observed in the most serious tissue with cervical lesions .fluorescence photometry corroborated these results. Conclusion: ALA is selectively accumlated in cervical lesions, and the more severe the lesion, the easier it is to accumulate.