Fluorescence microscopy is a powerful tool which enables to observe a large number of processes at the level of single cells. A protein of interest could be selectively labeled using fluorescent proteins, thereby allowing direct observation of its accumulation in living cells. This work is devoted to study a restriction-modification system which is a protective system of bacteria against foreign DNA. The action of such a system is based on DNA degradation by an enzyme - restriction endonuclease - and is strongly regulated to prevent host cell DNA damaging. In current study, the restriction-modification system contains controller protein, which regulates amount of restriction-modification system enzymes. Changes in amount of restriction-modification system proteins may cause a change in protection level of bacteria against viruses. We use fluorescence microscopy for direct observation of restriction-modification protein levels and their changes depending on the amount of controller protein in bacterial cells.
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