Optimized Raman Setting of Objective Lens, Laser Power and Integration Time for High and Low Concentration of Nonstructural Protein 1

摘要

Surface Enhanced Raman Spectroscopy (SERS) is a powerful analysis technique for detection of biological molecules. The quality of Raman spectra is highly dependent on the concentration of the molecules and the setting of Raman equipment. This paper describes the optimization of Raman setting which are the microscope objective lens, the laser power and the integration time for detection of non-structural protein 1 (NS1). NS1 protein is a biomarker for diseases related to flavivirus infection such as Dengue Fever (DF), Murray Valley Encephalitis (MVE), Yellow Fever (YF), Tick-borne Encephalitis (TBE), West Nile Encephalitis (WNE) and Japanese Encephalitis (JE). Optimum Raman setting for high (10ppm) and low (O.lppm) concentration of diluted NS1 are determined. From experiments, it shows that objective lens of 50X, laser power of ISOmW and integration time of 30sec, produces Raman spectra with highest peak intensity, at high concentration of NS1. With the same objective lens and integration time, laser power of 300mW is observed to produce highest peak intensity, at low concentration of NS1.