Biomass is a potential source of cellulose contained in nature in abundant amounts of either agricultural waste or plant. Corncob is one of biomass derived from waste of agricultural. High content of cellulose from corn cobs have the potential to be processed into glucose which is then fermented to produce bioethanol. Lignin contained in the corn cobs must be removed to produce high glucose levels, through choline acetate as ionic liquid in pretreatment process. Pretreatment process was done by adding choline acetate (ChoAc) as ionic liquid (IL) with a certain ratio between biomass to IL. The pretreatment was carried out at 90°C, 120°C and 150°C of temperature with a reaction time of 20 hours. The highest lignin reduction and index of crystallinity (CrI) is 36.05% and 25.74% respectively, obtained in a pretreatment that uses 0.3 of biomass to IL ratio and 150°C of temperature. This reduction of lignin was aimed to obtain high yield bioethanol in the fermentation process due to high yield of glucose. And the greater the crystallinity index, the easier the enzymes or microorganisms will degrade glucose into bioethanol.
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