Portable CGE-SSCP lab-in-a-suitcase instrument for rapid determination of pathogenicity of avian influenza virus

摘要

A new solution for rapid identification of genetic markers of highly-pathogenic variant of avian influenza virus (HPAI) in a heterogeneous sample, utilizing portable lab-in-a-suitcase instrument with capillary gel electrophoresis method and single strand conformation polymorphism technique (CGE-SSCP) is presented. For the first time, minor highly pathogenic variant of avian flu has been distinguished at the background of dominating low-pathogenic strain in a few minutes (for standard PCR: > 2 hours), utilizing mobile instrument for genetic analysis which can be easily transported into the area of infection.