Use of waste from the leather industry for the production of biotechnological products based on collagen

摘要

Leather industry generates vast quantities of by-products which could be a cheap and available source of collagen and collagen peptides.These peptides have been widely used in cosmetic,biomedical,and pharmaceutical industries.The main objective of present study was to obtain a biotechnological product based on collagen and to study its properties.Lime fleshings from hides were fragmented into small pieces with size<0.5 cm.Collagen was extracted from trimming waste using 0.5 M acetic acid in the presence of 5 mM EDTA.The purity of extracted collagen was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE).Collagen was enzymatically hydrolyzed by pepsin.To stop the reaction,the mixture was heated immediately at 100°C and then centrifuged at 4000 g for 30 min.The supernatant was filtered through ceramic membrane for separation of collagen peptides fraction(26≤kDa).Collagen peptides were prepared from the obtained collagen.The generated collagen peptides was analyzed by SDS-PAGE using 18 % resolving gel.The influence of collagen peptides on antioxidant enzymes and lipid peroxidation were explored.Collagen peptides supplementation also lowered serum level of pro-inflammatory cytokines such as IL-1β,IL-12,while level of anti-inflammatory cytokine IL-4 was within the control value.