Towards An Active Micropump-mixer for Rapid Anti-platelet Drug Screening in Whole Blood

摘要

This work reports on development and characterization of an on-chip microfluidic handling system for application inpreclinical anti-platelet drug screening. A reciprocating elastomeric micropump/mixer design is presented for use withwhole human blood, utilizing flexible structural and actuation properties to manage hemodynamics for an on-chipplatelet thrombosis assay on fibrillar collagen.The hemocompatibility of the design is assessed across a range of operational configurations, demonstrating equivalentor superior performance to common microcapillary systems at a range of physiologically relevant shear conditions.Surprisingly efficient mixing phenomena are briefly investigated, validated using dyes within the molecular weight rangeof common antiplatelet therapies. Finally, a proof-of-concept preclinical application is explored, demonstrating that thisprototype can act as a real-time assay of anti-platelet drug pharmacokinetics, compared to an equivalent microcapillarysystem.