THE DEVELOPMENT OF SCALABLE BIOREACTOR SERIES FOR HUMAN INDUCED PLURIPOTENT STEM CELL STIRRED SUSPENSION CULTURE

摘要

Induced pluripotent stem (iPS) cells are promising cell sources for regenerative medicine. The stirred suspension culture using a bioreactor system is an efficient method for the large scale expansion of human iPS cells. On the basis of our hypothesis that a low share stress and the uniform medium flow in a vessel are important for the suspension culture of human iPS cells, we showed the bioreactor system which is capable of the stirred suspension culture of the inoculated single iPS cells in the aggregates condition at the last this conference. We designed the bioreactor which expanded culture volume to 5L, and tried scale up culture from 100mL this time.