Pulse laser activated uniform transfection and massively parallel intracellular delivery with high efficiency and high cell viability

摘要

With the increasing need of regenerative medicines, cell therapy, and biopharmaceutical drug, it is necessary to develop a method for massively parallel delivery and uniform transfection. This research explores the possibility of using μCP (using cell adhesive and cell blocking agents) for printing massive number of cells on a substrate and using optoporation methods (nanc-second pulsed radiation) to perforate cell membranes. With combining these two methods, a stand-alone micro-device capable of performing localized intracellular delivery will be developed.