UTILITY OF PARR ANALYSIS FOR IMPROVED DETECTION OF LYMPHOMA IN FELINE ENDOSCOPIC DUODENAL BIOPSIES

摘要

The purpose of this study was to assess the sensitivity and specificity of PARR (PCR for Antigen Receptor Rearrangement) analysis performed on feline endoscopic duodenal biopsies for detection of intestinal lymphoma, and to compare the sensitivity andspecificity of PARR analysis to routine histologic evaluation.A retrospective analysis of feline endoscopic Gl biopsies submitted to a commercial diagnostic laboratory was undertaken by identifying 190 sequential cases of feline endoscopic duodenal biopsy samples from the laboratory database over a five-month period. A survey was mailed to the submitting veterinarian to determine clinical progression following biopsy. Of 83 follow-up surveys received, information on clinical behavior was available for 75 cases. Median time from biopsy to survey completion was 3.7years and median age of the cats was 11.9 years. Analysis of the formalin-fixed paraffin-embedded duodenal biopsy samples for the 75 cases was performed by PARR to assess for clonality in TRG (T cell receptor gamma).