Effects of pulsed electromagnetic fields on the mRNA expression of RANKL and OPG in ovariectomized rat osteoblast-like cell

摘要

This study was designed to determine the effects of pulsed electromagnetic fields (PEMFs) on the mRNA expression of the receptor activator of NF-kJB ligand (RANKL) and osteoprotegerin (OPG) in ovariectomized rat osteoblast-like cells. Osteoblast-like cells were harvested from calvariae of rats, from which the ovaries had been totally excised. The third passaged osteoblast-like cells were used in the present experiments. Osteoblast-like cells were exposed to PEMFs for 5 days with 3.8 mT, 8 Hz, and 40 min per day. The expression of RANKL and OPG mRNA was determined with real-time fluorescent quantitative polymerase chain reaction. Compared with the sham group, the level of serum estradiol in the ovariectomized group was significantly decreased (P<0.05). Compared with the ovariectomized experimental (PEMFs) group and sham group, RANKL mRNA expression was significantly increased in the ovariectomized group (P<0.05, P<0.01, respectively). These data suggest that PEMFs could regulate the expression of RANKL mRNA of osteoblast-like cells.