Development of Renewable RNA Reference Standards for Non-Small Cell Lung Carcinoma (NSCLC) Diagnostics

摘要

A large number of lung cancers - the most common and lethal tumours in both men and women- are caused by genomic re-arrangements involving the ALK, RET and ROS genes, which can be treated with Crizotinib, resulting in improved patient survival. Fluorescence in-situ hybridisation (FISH) and Immuno-histochemistry have been the historical gold-standard diagnostic approaches for lung cancers involving fusions. However, these methods are technically demanding, their turn-around time is long and the interpretation of the results is challenging, even to the expert operator. In contrast, RNA workflows such as RT-PCR and targeted RNA-seq are being increasingly used in diagnostics to detect the cancer fusion transcripts because of their accuracy, precision and simpler interpretation.