Combinatorial Therapy of Rolipram and pNGF for Traumatic Brain Injury

摘要

We demonstrated that PgP polymeric micelle can be a promising non-viral gene carrier for pNGF to B35 neuroblastoma cells in both non-serum and 10% serum condition. We also demonstrated persistent elevation of NGF expression over a course of 5 days. The hydrophobic drug rolipram was loaded in PgP the loading efficiency was about 86%. Currently, we are preparing to transfect PgP/pNGF polyplexes in microglia C8-B4 (ATCC CRL-2540) cells to model transfection of immune cells that would be present at the site of injury. In the future, we will evaluate the synergistic efficiency of rolipram loaded PgP/pNGF in C8-B4 microglia cells in gaseous and CoCl_2 induced hypoxia condition in vitro.