Poly(beta-amino ester) nanoparticles for selective and combinatorial delivery of siRNA to brain cancer

摘要

We have been able to synthesize novel, bioreducible PBAE nanoparticles that are capable of safe and effective siRNA delivery to primary human brain cancer cells. Bioreducible PBAEs encapsulate siRNA into nanoparticles, but quickly and completely release siRNA within minutes when exposed to an environment comparable to the reducing cytoplasm. We have shown that these nanoparticles can selectively deliver siRNA to human brain cancer cells while avoiding transfection of noncancer brain cells. Finally, we have been able to show that we can create nanoparticles that have the potential to deliver several types of siRNA simultaneously while achieving near-complete knockdown of each targeted gene. These results suggest that PBAEs have the potential to selectively transfect brain cancer and target multiple genes at once.