Effects of Self-assembling Peptide RADA16-I Hydrogel on Neural Differentiation of Bone Marrow Mesenchymal Stem Cells

摘要

To establish composite culture system of rat bone marrow mesenchymal stem cells (BMSCs) and self assembling peptide hydrogel RADA16-I, and to investigate the effect of RADA16-I hydrogel on neural differentiation of BMSCs. BMSCs were isolated, cultivated and labeled with green fluorescent protein (GFP), then they were inoculated on glass coverslips or in RADA16-I solution to form control group and RADA16-I group respectively. The morphological changes of BMSCs induced by neural induction medium were observed, and GFAP, NeuN and Map-2 expressions of BMSCs in each group were detected with immunofluorescence. The induced BMSCs presented neuron-like change, and the rates of GFAP and NF-200 positive cells in RADA16- I group were higher than that in control group (P < 0.05). Self assembling peptide RADA16-I hydrogel can promote neural differentiation of BMSCs, and which may be used as scaffold material on BMSCs transplantation for treatment of nervous system diseases.