Preparation and in Vitro Release of Alginate/Chitosan Microparticles Containing α-glucosidase Inhibiting Peptide

摘要

Alginate-chitosan multilayer beads containing α-glucosidase inhibiting peptide (GIP) from Ruditapes philippinarum were prepared to develop stable, non-toxic microcapsules. The optimal conditions were alginate concentration (w/v) 1.0%, alginate/GIP ratio (w/w) 1:5, calcium chloride concentration (w/v) 5% and chitosan concentration (w/v) 2.0%, which were obtained through single factor tests and orthogonal experiments. Under the conditions, the encapsulation efficiency (EE%) was 67.21% and the drug-loading percentage was 33.67%．According to the release study, the release rate of GIP was lower than 30% after incubation in simulated gastric fluid (SGF) for 3h and the rate was higher than 70% after incubation in simulated intestinal fluid (SIF) for 5h. The inhibiting rate was 25.13% after treatment in SIF when the concentration of GIP was 10mg/mL. Accordingly，the microcapsules prepared in this paper are acid resistant and therefore can be given orally.