Taylor Dispersion: A Tool for Mass-Weighted, UV Selective Analysis of Hydrodynamic Size in Complex Formulations

摘要

Taylor Dispersion is a theoretical model that describes the behaviour of a sample plug traversing a cylindrical pipe under Poiseuille flow [1]. The combination of dispersive and diffusive properties of the molecules contained within the sample plug give rise to characteristic flow profiles - known as Taylorgrams - from which the molecular diffusion coefficient and, in turn, hydrodynamic radius can be determined. Using data acquired with the Viscosizer 200 system (Malvern Instruments, Worcestershire UK) -which couples a microcapillary with UV detection to visualise the flow profiles of nanolitre sample pulses - we show, with particular examples, the characteristic Taylorgrams of small molecules, proteins and samples with multi-component species.