Non-washing layer-by-layer assembly allows to obtain concentrated (3-5 mg/mL) colloids of 150-200 nm diameter capsules encasing poorly soluble drug nanocrystals or soft gel-like nanoparticles. Aggregation of the nanoparticles is prevented by using low molecular weight block-copolymers of poly(amino acids) with polyethylene glycol (PEG) in a combination with heparin and bovine serum albumin at every bilayer building step. Minimal amounts of the polyelectrolytes are used to recharge the surface of nanoparticles in the non-washing LbL process. Such PEGylated shells result in drug nanocapsules with a high colloidal stability in PBS buffer and increased protein adhesion resistance.
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