EFFECTS OF L-CARNITINE ENRICHMENT ON THE L-CARNITINE BIO-ENDOGEN OF THE MARINE ROTIFER Brachionus plicatilis AND THE CLODECERANS {Daphnia magna, Daphnia longispina AND Moina micrura)

摘要

In this study, marine rotifer B. plicatilis (0.1- 1000 mg L-carnitine per 1) and Cladocerans which are D. magna (0.1-100 mg L-carnitine per 1), D. longispina (0.01-10 mg L-carnitine per 1) and M. micrura (0.1-100 mg L-carnitine per 1) were enriched with varying levels (0, 0.01,0.1,1,10,100 and 1000 mg L-carnitine per 1) of L-carnitine supplements dissolved in culture medium. The enrichment trials were conducted 1.5 x 10~6cell ml~(-1) in Chlorella vulgaris density under an axenic condition in the laboratory. L-carnitine enrichment has shown considerable influence on the L- carnitine bio-endogen of rotifer and cladocerans. The highest L-carnitine content was detected in 1000 mg l~(-1) L-carnitine-treated rotifers. L-carnitine contents of cladoceran (D. magna and M, micrura) were 100 mg l"1 L-carnitine whereas L- carnitine content of D. longisipina was 10 mg l~(-1) L-carnitine. The results suggest that L-carnitine supplementation could be a positive factor to enhance on L-carnitine endogen of live foods.