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Cloning and sequence analysis of ribosomal protein L13 gene (rpL13) from Ailuropoda melanoleuca

机译:核糖体蛋白L13基因(RPL13)的克隆及序列分析来自Ailuropoda melanoleca

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To explore the structure characteristic of rpL13 gene of Ailuropoda melanoleuca, RT-PCR and PCR strategy were employe d to isolate cDNA and genomic sequence of this gene from A. melanoleuca respectively, followed by sequencing and analyz ing preliminarily. The results indicated that the length of cDNA fragment cloned was 660 bp, containing an open reading frame of 636 bp, encoding 211 amino acids of deduced protein with an estimated molecular weight of 24.29 KD and PI of 12.17. The length of the genomic sequence is 1831 bp, with five exons and four introns. Alignment analysis indicate d that rpL13 is highly similarity with the reported species both at the level of DNA and protein. The predicted protein possessed 3 different patterns of functional sites. The results presented here will enrich the rpL13 gene database.
机译:为了探讨厌氧素丝氨酸的RPL13基因的结构特征,RT-PCR和PCR策略分别将该基因的cDNA和基因组序列分别分别分离,然后初步测序和分析。结果表明,克隆的cDNA片段的长度为660bp,含有636bp的开放读数框,编码推导的蛋白质的211氨基酸,其分子量为24.29kd和pi为12.17。基因组序列的长度为1831bp,具有五个外显子和四个内含子。对准分析表明,在DNA和蛋白质的水平下,RPL13与报告的物种具有高度相似性。预测的蛋白质具有3种不同的功能位点模式。此处呈现的结果将丰富RPL13基因数据库。

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